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We’ll be back soon with another exciting gallery as well. We’ve attached the full gallery of over 70 images to this post, but you can also check out a full review in the gallery thread by clicking “Discuss on the TokuNation Forums” at the bottom of this post. His alternate claw hands, the Giga Battle Nizer in 2 configurations, and even specific effect parts for the Belial Shot & Belial Deathscythe attacks! Frankly this is an incredible release for the time. Actually Belial even has a really great set of accessories. While not perfect, Belial has a surprising amount of personality and is an intricately detailed figure. One of the oldest Ultra-Act releases in the line, in fact the 2nd, Ultraman Belial still holds up pretty well all these years later. So given Geed’s popularity we wanted to do a throwback gallery to the original Ultra-Act Ultraman Belial! Despite his prominence Belial has only ever received a couple of really nice collector oriented figures. 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Linear assembled construct digested by BamH gel results All samples were linearized with BamHI-HF beforehand.įigure4. HydA and PsbA1 assembly gel resultsĪfter transformation of the assembled construct into the DH5alpha cloning strain, DNA extraction results illustrated bands higher than empty vector p233 (also known as MF (Maturation Factor)). As it can be seen from Figure 1 and Figure 2 successful amplification of genes of interest was verified via gel electrophoresis.įigure1: PsbA1 amplification gel results.Īmplified HydA and psbA1 genes were assembled via Polymerase Cycling Assembly (PCA) and also verified via gel electrophoresis as shown on Figure 3.įigure 3. To assemble psbA1 and HydA genes, firstly, amplification of psbA1 (503 bp) from p233(11830 bp) and HydA(1998 bp) from plasmid PAM (5004 bp) were done. Plasmid p233 that contains hydrogenase maturation factors HydEF and HydG under psbA1 promoter and HydA under psbA1 promoter in 0015 plasmids. Due to complications with amplification and assembly of synthetic sequences of genes that we ordered like complex stable secondary structures and a lot of long repeats we switched to using plasmids that we received from as a kind gesture from Daniel C. |
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